This table provides information required to read the output (excel) file provided to the customer, key characteristics of the separate biomarkers and method description, if published.

The second column lists the names in the header of the first row of the excel sheet. These abbreviations are for some biomarkers a short version of the more informative abbreviations used in ”Platforms”, ”Prices” and ”About the biomarkers”, which are given in parenthesis, first column. In the excel sheet, the (short) abbreviation is followed by the actual platform. Thus, the designation tHcy A (tHcy(space)A) means tHcy measured at platform A.
Platform A- GC-MS
Abbr PubChem
CID
Unit Reported values LOD CV (%)
Wtn-d/Btw-d
ICC Publ*
Methylmalonic acid MMA 487 µmol/L < 0.26 0.03 1-4 3-8 0.81
Total homocysteine tHcy 91552 µmol/L 5-15 0.1 1-2 2 0.72
Total cysteine tCys 5862 µmol/L 150-350 0.1 1 -2 4-8 0.62
Methionine Met 6137 µmol/L 18-33 1 1 3 0.33
Serine Ser 5951 µmol/L 95-125 10 2 3-6 0.71
Glycine Gly 750 µmol/L 200-300 3 1-2 3 0.81
Platform B- GC-MS/MS
Abbr PubChem
CID
Unit Reported values LOD CV (%)
Wtn-d/Btw-d
ICC Publ*
Methylmalonic acid MMA 487 µmol/L < 0.26 0.03 2 3 0.81
Total homocysteine tHcy 91552 µmol/L 5-15 0.1 1 2 0.72
Total cysteine tCys 5862 µmol/L 150-350 0.1 1 2 0.62
Methionine Met 6137 µmol/L 20-50 1 1 3 0.33
Serine Ser 5951 µmol/L 95-200 10 2 3 0.71
Glycine Gly 750 µmol/L 150-300 3 1 3 0.81
Cystathionine Cysta 439258 µmol/L 0.07-0.55 0.05 2 6 0.63
Sarcosine Sarc 1088 µmol/L 0.7-2.3 0.035 1.5 1.6 0.68
Histidine His 6274 µmol/L 40-200 1 2 3 na
Tryptophan Trp 6305 µmol/L 43–89 0.1 2 2 0.51
Kynurenine Kyn 161166 µmol/L 1.6-2.9 0.12 2 3 0.68
3-Hydroxykynurenine (urine) HK 11811 np
Kynurenic acid (urine) KA 3845
Anthranilic acid (urine) AA 227
Xanthurenic acid (urine) XA 5699
3-Hydroxyanthranilic acid (urine) HAA 86
Pyridoxic acid (urine) PA 6723
Formiminoglutamic acid (urine) Figlu 439233
Ornithine Orn 6262 µmol/L 30-90 1 2 2
Aspartic acid Asp 5960 µmol/L 0-26 0.5 1.8 4
Glutamic acid Glu 33032 µmol/L 20-140 0.5 2 2.5
Lysine Lys 5962 µmol/L 120-290 1.2 2 3
Alanine Ala 5950 µmol/L 230-510 1 1.5 1.7
Phenylalanine Phe 6140 µmol/L 26-85 0.5 1.4 3
Isoleucine Ile 6306 µmol/L 40-140 0.5 2 2
Leucine Leu 6106 µmol/L 70-170 0.5 2.2 2.2
Proline Pro 145742 µmol/L 110-360 0.5 2.1 2.5
Valine Val 6287 µmol/L 150-350 0.5 2.9 2.3
Asparagine Asn 6267 µmol/L 20-130 0.6 3 3
Glutamine Gln 5961 µmol/L 390-700 10 2 2.5
Threonine Thr 6288 µmol/L 70-240 1.2 2.7 2.6
Tyrosine Tyr 6057 µmol/L 40-110 0.5 1.9 3.2
α-Ketoglutaric acid aKG 51 µmol/L 20-50 0.4 3.5 3.3
3-Hydroxyisoburyrate 3HIB 11217234 µmol/L 15-25
Platform C- LC-MS/MS
Abbr PubChem
CID
Unit Reported values LOD CV (%)
Wtn-d/Btw-d
ICC Publ*
Choline Chol 305 µmol/L 5 –12 0.5 5-8 4-7 0.36
Total choline tChol mmol/L 2-3 0.5 np
Trimethylamineoxide TMAO 1145 µmol/L na
Betaine Bet 247 µmol/L 16–51 0.5 6-7 8-10 0.65
Dimethylglycine DMG 673 µmol/L 1.5–4 1.25 3 5 0.64
Total homocysteine tHcy 91552 µmol/L <15 0.1 1 2 0.72
Creatine Crn 586 µmol/L na np
Creatinine Creat 588 µmol/L <90 0.25 3-5 2-6 0.79
Methionine Met 6137 µmol/L 18–33 1 4-7 3-7 0.33
Methionine sulfoxide MetSo 158980 µmol/L < 0.5 0.06 9-12 8 0.36
Cystathionine Cysta 439258 µmol/L < 0.4 0.025 6-12 7-11 0.63
Total cysteine tCys 5862 µmol/L 150-350 0.1 2 6 0.56
Histidine His 6274 µmol/L 40-200 0.25 1.6-2.4 1.7-2.5 na np
3-Methylhistidine (3-MH) m3His 92105 µmol/L 0.7-14 0.6 3 5 na np
1-Methylhistidine (1-MH) m1His 64969 µmol/L 2.7-7 0.25 7 13 na np
Arginine Arg 6322 µmol/L 10-80 0.25 4-6 2-6 0.53
Asymmetric dimethylarginine ADMA 123831 µmol/L 0.5-0.8 0.08 5-7 7-9 0.53
Symmetric dimethylarginine SDMA 169148 µmol/L 0.4-0.8 0.08 8-9 6-9 0.62
Homoarginine hArg 9085 µmol/L 1.2-3.3 0.10 6-7 5-8 0.65
Trimethyllysine TML 440121 µmol/L 0.4-1.3 0.2 6-7 9-12 0.15
Platform D- LC-MS/MS
Abbr PubChem
CID
Unit Reported values LOD CV (%)
Wtn-d/Btw-d
ICC Publ*
Pyridoxal 5'-phosphate PLP 1051 nmol/L 17-102 0.2 3 6-7 0.70
Pyridoxal PL 1050 nmol/L 6-28 0.2 3-5 5-6 0.62
4-Pyridoxic acid PA 6723 nmol/L 11-88 0.5 3-4 7-11 0.58
Pyridoxamine PM 1052 nmol/L   0.1 7-9 13-15 na
Pyridoxine PN 1054 nmol/L   0.05 3-5 7 na
Thiamine Thi 1130 nmol/L 7-16         np
Thiamine monophosphate TMP 1131 nmol/L 1-3         np
Riboflavin Ribo 493570 nmol/L 5-38 0.2 6 11-13 0.79
Flavin mononucleotide FMN 643976 nmol/L 5-29 0.4 12-18 13-22 0.69
Cystathionine Cysta 439258 µmol/L < 0.4 0.005 2-3 5-6 0.63
Neopterin Neopt 4455 nmol/L < 20 0.7 3-5 6-10 0.60
Tryptophan Trp 6305 µmol/L 43-89 4 3-4 6 0.51
Kynurenine Kyn 161166 µmol/L 1.6-2.9 0.007 3-5 6-9 0.68
Kynurenic acid KA 3845 nmol/L 20-100 0.4 3-5 9-11 0.68
Anthranilic acid AA 227 nmol/L 7-30 0.7 2-3 5-9 0.64
3-Hydroxykynurenine HK 11811 nmol/L 10-65 2 4-5 10 0.62
Xanthurenic acid XA 5699 nmol/L 5-35 0.5 7-10 15-17 0.58
3-Hydroxyanthranilic acid HAA 86 nmol/L 7-50 2 3-5 12-13 0.54
Picolinic acid Pic 1018 nmol/L         na np
Quinolinic acid QA 1066 nmol/L 134-384 1.6 7-8 7-10 na
Nicotinic acid NA 938 nmol/L <20 20 10-17 11-15 na
Nicotinamide NAM 936 nmol/L 92-388 20 10-12 9-12 na
N1-methylnicotinamide mNAM 457 nmol/L 27-221 5 5-7 7-9 na
Cotinine Cot 854019 nmol/L <85 1 2-3 6 0.92
Trans-3'-hydroxycotinine OHCot 107963 nmol/L   7 3 3 0.85
Trigonelline Trig 5570 µmol/L   0.010 3 5 na np
Platform E- LC-MS/MS
Abbr PubChem
CID
Unit Reported values LOD CV (%)
Wtn-d/Btw-d
ICC Publ*
5-Methyl-tetrahydrofolate mTHF 439234 nmol/L > 5 0.07 5-9 4-5 na
5-Formyl-tetrahydrofolate fTHF 6006 nmol/L 0-2 0.52 8-9 6-11 na
Folic acid FA 6037 nmol/L 0-2 0.27 7-8 6-12 na
4-Alfa-hydroxy-5-methyl-THF hmTHF nmol/L ≤ mTHF 0.20 6-10 6-7 na
Para-aminobenzoylglutamate pABG 196473 nmol/L 0-0.5 0.08 6-9 3-10 na
Acetamidobenzoylglutamate apABG 3080868 nmol/L 0.01-1 0.13 5-6 5-9 na
Platform F- Microbiological assays
Abbr Unit Reported values LOD CV (%)
Wtn-day/Btw-day
ICC Publ*
Folate (s-Folate) spFolate nmol/L > 7.5 2 4 5 0.56
Cobalamin (vitamin B12, s-B12) Cob pmol/L > 150 30 4 5 0.82
Platform G- MALDI-TOF
Abbr Unit Reported values LOD CV (%)
Wtn-day/Btw-day
ICC Publ*
C-reactive protein (hsCRP) CRP µg/ml 0-10 0.1 3-6 3-7 na
Serum amyloid A (SAA) SAAt µg/ml 0-20       na np
Calprotectin (Calp) S100At µg/ml 0-30       na np
Cystatin C (Cys-C) CnCt µg/ml 0-4 0.003 3-9 5-8 na
Glycated hemoglobin HbA1c % 4-14 na 5 na na
Platform H- LC-MS/MS
Abbr PubChem
CID
Unit Reported values LOD CV (%)
Wtn-d/Btw-d
ICC Publ*
All-trans retinol (Vit. A) vA 445354 µmol/L 1.5-2.8 0.1 4 6-8 0.87
25-hydroxyvitamin-D2 (25OH-D2) vD2 5710148 nmol/L 0-4 3.3 4-5 5-8 na
25-hydroxyvitamin-D3 (25OH-D3) vD3 5283731 nmol/L 20-150 3.3 4-5 7-8 0.71
Alfa-tocopherol (Vit. E) aToc 14985 µmol/L 23-46 <10 2-4 4-5 0.86
Gamma-tocopherol (Vit. E) gToc 92729 µmol/L 0.9-8.7 na 3 4 na
Phylloquinone (Vit. K1) K1 5284607 nmol/L   0.33 6-8 8-17 na
Menaquinone-4 (Vit. K2) K2 5282367     na na na na np

Abbreviations: LOD, lower limit of detection; ICC, intraclass correlation coefficient; np, not published; na, not available.
*For some biomarkers the assay performance parameters may be different from those given in published articles. This is due to continuous improvements of the methods.