This table provides information required to read the output (excel) file provided to the customer, key characteristics of the separate biomarkers and method description, if published.

The second column lists the names in the header of the first row of the excel sheet. These abbreviations are for some biomarkers a short version of the more informative abbreviations used in ”Platforms”, ”Prices” and ”About the biomarkers”, which are given in parenthesis, first column. In the excel sheet, the (short) abbreviation is followed by the actual platform. Thus, the designation tHcy A (tHcy(space)A) means tHcy measured at platform A.
Platform A- GC-MS
Abbr Unit Reported values LOD CV (%)
Wtn-day/Btw-day
ICC Publ*
Methylmalonic acid MMA µmol/L < 0.26 0.03 1-4 3-8 0.81
Total homocysteine tHcy µmol/L 5-15 0.1 1-2 2 0.72
Total cysteine tCys µmol/L 150-350 0.1 1 -2 4-8 0.62
Methionine Met µmol/L 18-33 1 1 3 0.33
Serine Ser µmol/L 95-125 10 2 3-6 0.71
Glycine Gly µmol/L 200-300 3 1-2 3 0.81
Platform B- GC-MS/MS
Abbr Unit Reported values LOD CV (%)
Wtn-day/Btw-day
ICC Publ*
Methylmalonic acid MMA µmol/L < 0.26 0.03 2 3 0.81
Total homocysteine tHcy µmol/L 5-15 0.1 1 2 0.72
Total cysteine tCys µmol/L 150-350 0.1 1 2 0.62
Methionine Met µmol/L 20-50 1 1 3 0.33
Serine Ser µmol/L 95-200 10 2 3 0.71
Glycine Gly µmol/L 150-300 3 1 3 0.81
Cystathionine Cysta µmol/L 0.07-0.55 0.05 2 6 0.63
Sarcosine Sarc µmol/L 0.7-2.3 0.035 1.5 1.6 0.68
Histidine His µmol/L 40-200 1 2 3 na
Tryptophan Trp µmol/L 43–89 0.1 2 2 0.51
Kynurenine Kyn µmol/L 1.6-2.9 0.12 2 3 0.68
3-Hydroxykynurenine (in urine) HK np
Kynurenic acid (in urine) KA
Anthranilic acid (in urine) AA
Xanthurenic acid (in urine) XA
3-Hydroxyanthranilic acid (in urine) HAA
Pyridoxic acid (in urine) PA
Formiminoglutamic acid (in urine) Figlu
Ornithine Orn µmol/L 30-90 1 2 2
Aspartic acid Asp µmol/L 0-26 0.5 1.8 4
Glutamic acid Glu µmol/L 20-140 0.5 2 2.5
Lysine Lys µmol/L 120-290 1.2 2 3
Alanine Ala µmol/L 230-510 1 1.5 1.7
Phenylalanine Phe µmol/L 26-85 0.5 1.4 3
Isoleucine Ile µmol/L 40-140 0.5 2 2
Leucine Leu µmol/L 70-170 0.5 2.2 2.2
Proline Pro µmol/L 110-360 0.5 2.1 2.5
Valine Val µmol/L 150-350 0.5 2.9 2.3
Asparagine Asn µmol/L 20-130 0.6 3 3
Glutamine Gln µmol/L 390-700 10 2 2.5
Threonine Thr µmol/L 70-240 1.2 2.7 2.6
Tyrosine Tyr µmol/L 40-110 0.5 1.9 3.2
α-Ketoglutaric acid aKG µmol/L 20-50 0.4 3.5 3.3
3-Hydroxyisoburyrate 3HIB µmol/L 15-25
Platform C- LC-MS/MS
Abbr Unit Reported values LOD CV (%)
Wtn-day/Btw-day
ICC Publ*
Choline Chol µmol/L 5 –12 0.5 5-8 4-7 0.36
Total choline tChol mmol/L 2-3 0.5 np
Trimethylamineoxide TMAO µmol/L na
Betaine Bet µmol/L 16–51 0.5 6-7 8-10 0.65
Dimethylglycine DMG µmol/L 1.5–4 1.25 3 5 0.64
Total homocysteine tHcy µmol/L <15 0.1 1 2 0.72
Creatine Crn µmol/L na np
Creatinine Creat µmol/L <90 0.25 3-5 2-6 0.79
Methionine Met µmol/L 18–33 1 4-7 3-7 0.33
Methionine sulfoxide MetSo µmol/L < 0.5 0.06 9-12 8 0.36
Cystathionine Cysta µmol/L < 0.4 0.025 6-12 7-11 0.63
Total cysteine tCys µmol/L 150-350 0.1 2 6 0.56
Histidine His µmol/L 40-200 0.25 1.6-2.4 1.7-2.5 na np
3-Methylhistidine (3-MH) m3His µmol/L 0.7-14 0.6 3 5 na np
1-Methylhistidine (1-MH) m1His µmol/L 2.7-7 0.25 7 13 na np
Arginine Arg µmol/L 10-80 0.25 4-6 2-6 0.53
Asymmetric dimethylarginine ADMA µmol/L 0.5-0.8 0.08 5-7 7-9 0.53
Symmetric dimethylarginine SDMA µmol/L 0.4-0.8 0.08 8-9 6-9 0.62
Homoarginine hArg µmol/L 1.2-3.3 0.10 6-7 5-8 0.65
Trimethyllysine TML µmol/L 0.4-1.3 0.2 6-7 9-12 0.15
Platform D- LC-MS/MS
Abbr Unit Reported values LOD CV (%)
Wtn-day/Btw-day
ICC Publ*
Pyridoxal 5'-phosphate PLP nmol/L 17-102 0.2 3 6-7 0.70
Pyridoxal PL nmol/L 6-28 0.2 3-5 5-6 0.62
4-Pyridoxic acid PA nmol/L 11-88 0.5 3-4 7-11 0.58
Pyridoxamine PM nmol/L   0.1 7-9 13-15 na
Pyridoxine PN nmol/L   0.05 3-5 7 na
Thiamine Thi nmol/L 7-16         np
Thiamine monophosphate TMP nmol/L 1-3         np
Riboflavin Ribo nmol/L 5-38 0.2 6 11-13 0.79
Flavin mononucleotide FMN nmol/L 5-29 0.4 12-18 13-22 0.69
Cystathionine Cysta µmol/L < 0.4 0.005 2-3 5-6 0.63
Neopterin Neopt nmol/L < 20 0.7 3-5 6-10 0.60
Tryptophan Trp µmol/L 43-89 4 3-4 6 0.51
Kynurenine Kyn µmol/L 1.6-2.9 0.007 3-5 6-9 0.68
Kynurenic acid KA nmol/L 20-100 0.4 3-5 9-11 0.68
Anthranilic acid AA nmol/L 7-30 0.7 2-3 5-9 0.64
3-Hydroxykynurenine HK nmol/L 10-65 2 4-5 10 0.62
Xanthurenic acid XA nmol/L 5-35 0.5 7-10 15-17 0.58
3-Hydroxyanthranilic acid HAA nmol/L 7-50 2 3-5 12-13 0.54
Picolinic acid Pic nmol/L         na np
Quinolinic acid QA nmol/L 134-384 1.6 7-8 7-10 na
Nicotinic acid NA nmol/L <20 20 10-17 11-15 na
Nicotinamide NAM nmol/L 92-388 20 10-12 9-12 na
N1-methylnicotinamide mNAM nmol/L 27-221 5 5-7 7-9 na
Cotinine Cot nmol/L <85 1 2-3 6 0.92
Trans-3'-hydroxycotinine OHCot nmol/L   7 3 3 0.85
Trigonelline Trig µmol/L   0.010 3 5 na np
Platform E- LC-MS/MS
Abbr Unit Reported values LOD CV (%)
Wtn-day/Btw-day
ICC Publ*
5-Methyl-tetrahydrofolate mTHF nmol/L > 5 0.07 5-9 4-5 na
5-Formyl-tetrahydrofolate fTHF nmol/L 0-2 0.52 8-9 6-11 na
Folic acid FA nmol/L 0-2 0.27 7-8 6-12 na
4-Alfa-hydroxy-5-methyl-THF hmTHF nmol/L ≤ mTHF8 0.20 6-10 6-7 na
Para-aminobenzoylglutamate pABG nmol/L 0-0.5 0.08 6-9 3-10 na
Acetamidobenzoylglutamate apABG nmol/L 0.01-1 0.13 5-6 5-9 na
Platform F- Microbiological assays
Abbr Unit Reported values LOD CV (%)
Wtn-day/Btw-day
ICC Publ*
Folate (s-Folate) spFolate nmol/L > 7.5 2 4 5 0.56
Cobalamin (vitamin B12, s-B12) Cob pmol/L > 150 30 4 5 0.82
Platform G- MALDI-TOF
Abbr Unit Reported values LOD CV (%)
Wtn-day/Btw-day
ICC Publ*
C-reactive protein (hsCRP) CRP µg/ml 0-10 0.1 3-6 3-7 na
Serum amyloid A (SSA) tSAA µg/ml 0-20       na np
Calprotectin (Calp) tCalp µg/ml 0-30       na np
Cystatin C (Cys-C) CnCt µg/ml 0-4 0.003 3-9 5-8 na
Glycated hemoglobin HbA1c % 4-14 na 5 na na
Platform H- LC-MS/MS
Abbr Unit Reported values LOD CV (%)
Wtn-day/Btw-day
ICC Publ*
All-trans retinol (Vit. A) vA µmol/L 1.5-2.8 0.1 4 6-8 0.87
25-hydroxyergocalciferol (25OH-D2) vD2 nmol/L 0-4 3.3 4-5 5-8 na
25-hydroxycholecalciferol (25OH-D3) vD3 nmol/L 20-150 3.3 4-5 7-8 0.71
Alfa-tocopherol (Vit. E) aToc µmol/L 23-46 <10 2-4 4-5 0.86
Gamma-tocopherol (Vit. E) gToc µmol/L 0.9-8.7 na 3 4 na
Phylloquinone (Vit. K1) K1 nmol/L   0.33 6-8 8-17 na
Menaquinone-4 (Vit. K2) K2     na na na na np

Abbreviations: LOD, lower limit of detection; ICC, intraclass correlation coefficient; np, not published; na, not available.
*For some biomarkers the assay performance parameters may be different from those given in published articles. This is due to continuous improvements of the methods.