Biodiversity and Conservation
9 (9): 1261-1273, September 2000
Copyright © 2000 Kluwer Academic
Publishers
All rights reserved
Conservation of the Cedrus libani populations
in Lebanon: history, current status and experimental application of somatic
embryogenesis
S. Khuri
Department Agricultural Botany, School
of Plant Sciences, The University of Reading, Whiteknights P.O. Box 221,
Reading, RG6 6AS, UK
M.R. Shmoury
Faculty of Agricultural and Food Sciences,
The American University of Beirut, P.O. Box: 11-0236, Beirut, Lebanon
R. Baalbaki
Faculty of Agricultural and Food Sciences,
The American University of Beirut, P.O. Box: 11-0236, Beirut, Lebanon
M. Maunder
Conservation Projects Development Unit,
Living Collections Department, Royal Botanic Gardens, Kew, Richmond, TW9
1AE, UK
S.N. Talhouk
Faculty of Agricultural and Food Sciences,
The American University of Beirut, P.O. Box: 11-0236, Beirut, Lebanon
Abstract
Cedrus libani, the cedar of Lebanon, is a threatened conifer
native to the Levant. Over 4000 years of exploitation have resulted in the
fragmentation and degradation of the Lebanese cedar populations. Continued
urban and agricultural development in Lebanon adds to the difficulty of effective
conservation. Two protected areas have recently been established which contain
two of the more important forests: a cedar dominated forest in the Shouf region
and a mixed forest at Ehden. A number of other populations are protected by
ministerial decrees, and there is a need for rigorous management of all the
remaining populations. The application of in vitro techniques such
as somatic embryogenesis may assist in the conservation of this species. We
have produced somatic pro-embryos using immature zygotic tissue as explants
cultured on half-strength MS medium containing an auxin and a cytokinin (10
μM 2,4-D and 5 μ
M BAP). The application of somatic embryogenesis to the Lebanese cedar would
be in the propagation and preservation of selected genotypes, either those
from old growth provenance for use in restoration, or those with desirable
commercial or horticultural characteristics.
Keywords
Cedrus, in vitro conservation, Lebanon,
somatic embryogenesis
Article ID: 258407