Method(s): MALDI-TOF MS.
What is measured: Glycated hemoglobin (HbA1c).
What is HbA1c?
It is formed by non-enzymatically attachment of glucose to different amino groups of both 2 alpha and 2 beta chains of normal adult hemoglobin A (HbA). The relative amount of glucose bond to the N-terminal of the beta chain is measured as HbA1c. HbA1c is used to monitor long-term glycaemic control and treatment of diabetes.
Performance of the assay
Lower limit of detection (LOD): na.
Within-day CV, 5 %; between-day CV, na.
Assessment of diabetes.
Specimen, collection and processing
Matrix: Whole blood.
Volume: Minimum volume is 20 µL, but 100 µL is optimal and allows reanalysis.
Preparation and stability: The blood sample must be put on ice, and frozen. HbA1c is stable for one week at 4-8C. At
Transportation; for general instruction on transportation, click here.
Frozen, on dry ice.
Reference values, interpretation
Reference values: 4-6%.
In diabetes patients values can range from 6.5 up to 14%.
Intraclass correlation coefficient (ICC): na.