Method(s): MALDI-TOF MS (1).
What is C-reactive protein?
CRP belongs to the protein family of pentraxins, which is characterized by calcium dependent ligand-binding of five monomers of each 23kDa forming a radial symmetric ring. CRP is an acute phase reactant that responds as a sensitive, but non-specific marker for systemic inflammation. Elevated CRP (>10ug/ml) have been related to higher risks of cardiovascular events, metabolic syndrome and cancer (2). Therefore, hsCRP has become an important biomarker and confounder in clinical and epidemiological studies.
Performance of the assay
Lower limit of detection (LOD): 0.1 µg/ml.
Within-day CV, 3-6 %; between-day CV, na.
Assessment of inflammation.
Specimen, collection and processing
Matrix: Plasma and serum. CRP is stable for 3 years at -20
Volume: Minimum volume is 20 µL, but 100 µL is optimal and allows reanalysis.
Preparation: The blood sample must be centrifuged and the plasma/serum fraction put on ice, and frozen.
CRP is stable for 3 years at -20
Transportation; for general instruction on transportation, click here.
Frozen, on dry ice.
Reported values, interpretation
Reported values: 0-10 µg/ml.
Intraclass correlation coefficient (ICC): 0.54.
1. Meyer, K., and Ueland, P.M. (2014). Targeted Quantification of C-Reactive Protein and Cystatin C and Its Variants by Immuno-MALDI-MS. Anal Chem 86, 5807-814
2. Kushner, I., Rzewnicki, D., and Samols, D. (2006). What does minor elevation of C-reactive protein signify? Am J Med 119, 166.e17-e28.