Method(s): GC-MS/MS (1).
Performance of the assay (GC-MS/MS)
Lower limit of detection (LOD): 0.25 µmol/L.
Within-day CV: 1.6-2.4 %; between-day CV: 1.7-2.5 %.
Histidine has antiinflammatory and antioxidant effects, is inversely related to CRP, and is low in obesity and renal dysfunction. Histidine values may complement the metabolic profile in studies on inflammatory conditions, obesity, diabetes and renal dysfunction.
Specimen, collection and processing
Matrix: Serum or EDTA plasma.
Volume: Minimum volume is 50 µL, but 200 µL is optimal and allows reanalysis.
Preparation and stability: The blood sample must be centrifuged and the plasma/serum fraction put on ice, and frozen.
Transportation; for general instruction on transportation, click here.
Frozen, on dry ice.
Reported values, interpretation
Reported values: 40-200 µmol/L.
Intraclass correlation coefficient (ICC): NA
1. Midttun, Ø., McCann, A., Aarseth, O., Krokeide, M., Kvalheim, G., Meyer, K., and Ueland, P.M. (2016). Combined measurement of 6 fat-soluble vitamins and 26 water-soluble functional vitamin markers and amino acids in 50 μL of serum or plasma by high-throughput mass spectrometry. Anal Chem 88, 10427-436.