Platform C: Chol, (tChol), TMAO, Bet, DMG, tHcy, Creat, Crn, Met, MetSo, Cysta, tCys, His, 3-MH, 1-MH, Cit, Orn, Arg, ADMA, SDMA, hArg, TML
Performance of the assay
Lower limit of detection (LOD): 1 µmol/L.
Within-day CV: 1 %; between-day CV: 3 %.
Assessment of one-carbon status. Homocystinuria.
Specimen, collection and processing
Patient/subject: Prandial status affects concentration, which increases after a protein rich meal.
Matrix: Serum or EDTA plasma.
Volume: Minimum volume is 50 µL, but 200 µL is optimal and allows reanalysis.
Preparation and stability: Methionine is converted to methionine sulfoxide, even at low temperature and in samples frozen at
Transportation; for general instruction on transportation, click here.
Frozen, on dry ice.
Reported values, interpretation
Reported values: 20-50 µmol/L.
Intraclass correlation coefficient (ICC): 0.33.
1. Midttun, Ø., McCann, A., Aarseth, O., Krokeide, M., Kvalheim, G., Meyer, K., and Ueland, P.M. (2016). Combined measurement of 6 fat-soluble vitamins and 26 water-soluble functional vitamin markers and amino acids in 50 μL of serum or plasma by high-throughput mass spectrometry. Anal Chem 88, 10427-436.
2. Midttun, O., Kvalheim, G., and Ueland, P.M. (2013). High-throughput, low-volume, multianalyte quantification of plasma metabolites related to one-carbon metabolism using HPLC-MS/MS. Anal Bioanal Chem 405, 2009-017.