Sarcosine



Synonym: Monomethylglycine.
Method(s): GC-MS/MS (1).

What is measured on the same platform, click here.
Platform B: MMA, tHcy, tCys, Met, Ser, Gly, Cysta, Sarc, His, Trp, Kyn, (KTR), HK, KA, XA, AA, HAA, NA, PA, Figlu, Orn, Asp, Glu, Lys, Ala, Phe, Ile, Leu, Pro, Val, Asn, Gln, Thr, Tyr, aKG, 3HIB, aHB, bHB, AcAc

What is sarcosine?

Sarcosine is formed from dimethylglycine (dimethylglycine dehydrogenase) or by methylation of glycine (glycine-N-methyl transferase). It is degraded to glycine (sarcosine dehydrogenase).

Performance of the assay

Lower limit of detection (LOD): na.
Within-day CV: 5 %; between-day CV: na.

Indication(s)

Assessment of choline and one-carbon status.

Specimen, collection and processing

Patient/subject: No special precaution.
Matrix: Sarcosine cannot be determined in samples collected into EDTA Vacutainer Tubes from some producer(s) (Becton, Dickinson), because of the presence of significant amounts of sarcosine in such tubes.
Volume: Minimum volume is 50 µL, but 200 µL is optimal and allows reanalysis.
Preparation and stability: The blood sample must be centrifuged and the plasma/serum fraction put on ice, and frozen.

Transportation; for general instruction on transportation, click here.

Frozen, on dry ice.

Reported values, interpretation

Reported values: 0.7-2.3 µmol/L
Intraclass correlation coefficient (ICC): 0.68.

Literature

1. Midttun, Ø., McCann, A., Aarseth, O., Krokeide, M., Kvalheim, G., Meyer, K., and Ueland, P.M. (2016). Combined measurement of 6 fat-soluble vitamins and 26 water-soluble functional vitamin markers and amino acids in 50 μL of serum or plasma by high-throughput mass spectrometry. Anal Chem 88, 10427-436.