Vitamin A

Method(s): LC-MS/MS.
What is measured: All-trans retinol.

What is measured on the same platform, click here.
Platform H: Vitamins A, D, E and K

Performance of the assay

Lower limit of detection (LOD): 0.1 µmol/L.
Within-day CV: 4 %; between-day CV: 6 - 8 %.


All-trans retinol is a marker of vitamin A status.

Specimen, collection and processing

Preferred matrix: EDTA plasma.
Volume: Minimum volume is 50 µL, but 200 µL is optimal and allows reanalysis.
Preparation: Serum gel tubes must not be used. Samples should be put on ice, and protected from light.

Transportation; for general instruction on transportation, click here.

Frozen, on dry ice.

Reported values, interpretation

Reported values: 1.5-2.8 µmol/L.
Intraclass correlation coefficient (ICC): 0.87.