KJEM230, Chromatographic animations


Diffusion

  1. Brownian motion

  2. Diffusion


Basic principle of Chromatography

  1. Extraction

  2. Repeated extraction

  3. Repeated extraction with higher mixing frequency

  4. Chromatography


Factors for resolution, the Purnell equation

  1. Sufficient resolution

  2. Reduced efficiency, N

  3. Reduced selectivity, α

  4. Reduced retention, k


Zone broadening, the Golay equation and optimal velocity

  1. Very low mobile phase velocity, u = 50 mm/s

  2. Low mobile phase velocity, u = 100 mm/s

  3. Moderate mobile phase velocity, u = 200 mm/s

  4. High mobile phase velocity, u = 400 mm/s

  5. Very high mobile phase velocity, u = 800 mm/s

  6. The resulting optimal velocity